Questions Addressed
How the machine learning algorithms can be used
to predict the amino acid substitution of a protein sequence so that
it will obtain higher activity and stability?
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What is machine
learning?
A field of artificial intelligence in which the algorithms were
used to improve the quality of the subject by learning through
past experience
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How are machine
learning algorithms used in our project?
In vivo
incorporation of non-natural amino acids can be used to improve
protein stability. However, there is a trade off; improved
stability of the protein may lead to loss in activity. One
way to improve function is to employ machine-learning algorithms
to identify the variants that improve activity. With the aid of computer guidance, we plan to design a set
of fluorinated variants for our target protein Tetrahymena GCN5
(tGCN5). Using information from biochemical and
structural data, we identified eleven residues to mutate. One of
the supervised machine learning algorithms, Linear Regression
Analysis, will be used to identify the residues that have positive
impact on the mutated sequences.
MRO-W
2007-2008 Mid-Year Progress Report
MRO-W
2007-2008 Final Report
