STEP 1. You need two protein sequences. They can be in either GENTRANS format or Matcher format. GENTRANS format: > m5 (1-80) AVTRGTINDPQRAKEALDKY ELENHDLKTKNEGLKTENEG LKTENEGLKTENEGLKTEKK EHEAENDKLKQQRDTLSTQK Matcher format: Seq_name Score Seq Path Ratio =========================== ===== === ==== ===== M5 (1-44) 1603 450 361 80% a b c d e f g 1 Ala Val Thr Arg Gly Thr Ile Asn Asp Pro 11 Gln Arg Ala Lys Glu Ala 17 Leu Asp Lys Tyr Glu Leu Glu 24 Asn His Asp Leu Lys Thr Lys 31 Asn Glu Gly Leu Lys Thr Glu 38 Asn Glu Gly Leu Lys Thr Glu STEP 2. Select a 2D structure and the minimal size for clusters reported. By selecting Coiled-coil, only amino acids on the surface of the protein (positions b, c, e, f, g) will be matched. Click here for more information. STEP 3. Enter the protein sequences in the appropriate fields. To see an example select one of the following.